Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication.
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ABSTRACT:
SUBMITTER: de Borba L
PROVIDER: S-EPMC4337542 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
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Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication.
de Borba Luana L Villordo Sergio M SM Iglesias Nestor G NG Filomatori Claudia V CV Gebhard Leopoldo G LG Gamarnik Andrea V AV
Journal of virology 20150114 6
The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances v ...[more]
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