Metabolic reprogramming in mutant IDH1 glioma cells.

ABSTRACT:

Background

Mutations in isocitrate dehydrogenase (IDH) 1 have been reported in over 70% of low-grade gliomas and secondary glioblastomas. IDH1 is the enzyme that catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate while mutant IDH1 catalyzes the conversion of α-ketoglutarate into 2-hydroxyglutarate. These mutations are associated with the accumulation of 2-hydroxyglutarate within the tumor and are believed to be one of the earliest events in the development of low-grade gliomas. The goal of this work was to determine whether the IDH1 mutation leads to additional magnetic resonance spectroscopy (MRS)-detectable changes in the cellular metabolome.

Methods

Two genetically engineered cell models were investigated, a U87-based model and an E6/E7/hTERT immorta

SUBMITTER: Izquierdo-Garcia JL 

PROVIDER: S-EPMC4338038 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature