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Single-point mutation detection in RNA extracts using gold nanoparticles modified with hydrophobic molecular beacon-like structures.

ABSTRACT: Gold nanoparticles functionalized with oligonucleotides that bear a cholesterol group are used as gene sensors. The hydrophobic molecule is buried inside the nanostructure but when the complementary RNA sequence is present the structure unfolds exposing the cholesterol group to the water molecules. This rearrangement leads to the aggregation of the nanostructures.

SUBMITTER: Latorre A

PROVIDER: S-EPMC4339280 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Single-point mutation detection in RNA extracts using gold nanoparticles modified with hydrophobic molecular beacon-like structures.

Latorre Alfonso A Posch Christian C Garcimartín Yolanda Y Ortiz-Urda Susana S Somoza Álvaro Á

Chemical communications (Cambridge, England) 20140301 23

Gold nanoparticles functionalized with oligonucleotides that bear a cholesterol group are used as gene sensors. The hydrophobic molecule is buried inside the nanostructure but when the complementary RNA sequence is present the structure unfolds exposing the cholesterol group to the water molecules. This rearrangement leads to the aggregation of the nanostructures. ...[more]

PMID: 24496380

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