Ontology highlight
ABSTRACT:
SUBMITTER: Panja S
PROVIDER: S-EPMC4340055 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Methods in molecular biology (Clifton, N.J.) 20150101
Fluorescence spectroscopy is a sensitive technique for detecting protein-protein, protein-RNA, and RNA-RNA interactions, requiring only nanomolar concentrations of labeled components. Fluorescence anisotropy provides information about the assembly of multi-subunit proteins, while molecular beacons provide a sensitive and quantitative reporter for base pairing between complementary RNAs. Here we present a detailed protocol for labeling Hfq protein with cyanine 3-maleimide and dansyl chloride to s ...[more]