Measuring protein reduction potentials using 15N HSQC NMR spectroscopy.
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ABSTRACT:
SUBMITTER: Taylor SL
PROVIDER: S-EPMC4340554 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Measuring protein reduction potentials using 15N HSQC NMR spectroscopy.
Taylor Samantha L SL Crawley-Snowdon Harriet H Wagstaff Jane L JL Rowe Michelle L ML Shepherd Mark M Williamson Richard A RA Howard Mark J MJ
Chemical communications (Cambridge, England) 20130129 18
NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple (15)N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a. ...[more]
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