Unknown

Dataset Information

0

Three novel cis-acting elements required for efficient plus-strand DNA synthesis of the hepatitis B virus genome.


ABSTRACT: Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. To determine cis-acting elements apart from the donor and acceptor sites that are required for plus-strand RC DNA synthesis by hepatitis B virus (HBV), a series of mutants bearing a small deletion were made and analyzed for their impact on the viral genome synthesis. We found three novel cis-acting elements in the HBV genome: one element, located in the middle of the minus strand, is indispensable, whereas the other two elements, located near either end of the minus strand, contribute modestly to the plus-strand RC DNA synthesis. The data indicated that the first element facilitates plus-strand RNA primer translocation or subsequent elongation during plus-strand RC DNA synthesis, while the last two elements, although distantly located on the minus strand, act at multiple steps to promote plus-strand RC DNA synthesis. The necessity of multiple cis-acting elements on the minus-strand template reflects the complex nature of hepadnavirus reverse transcription.

SUBMITTER: Lee J 

PROVIDER: S-EPMC434075 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Three novel cis-acting elements required for efficient plus-strand DNA synthesis of the hepatitis B virus genome.

Lee Jehan J   Shin Myeong-Kyun MK   Lee Hye-Jin HJ   Yoon Gyesoon G   Ryu Wang-Shick WS  

Journal of virology 20040701 14


Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. To determine cis-acting elements apart from the donor and acceptor sites that are required for plus-strand RC DNA synthesis by hepatitis B virus (HBV), a series of mutants bearing a smal  ...[more]

Similar Datasets

| S-EPMC4147681 | biostudies-literature
| S-EPMC1900078 | biostudies-literature
| S-EPMC4031142 | biostudies-literature
| S-EPMC7239442 | biostudies-literature
| S-EPMC5553863 | biostudies-literature
| S-EPMC3346311 | biostudies-literature
| S-EPMC1075688 | biostudies-literature
| S-EPMC3004326 | biostudies-literature
| S-EPMC4096559 | biostudies-literature
| S-EPMC6161694 | biostudies-literature