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Precision assembly of complex cellular microenvironments using holographic optical tweezers.


ABSTRACT: The accurate study of cellular microenvironments is limited by the lack of technologies that can manipulate cells in 3D at a sufficiently small length scale. The ability to build and manipulate multicellular microscopic structures will facilitate a more detailed understanding of cellular function in fields such as developmental and stem cell biology. We present a holographic optical tweezers based technology to accurately generate bespoke cellular micro-architectures. Using embryonic stem cells, 3D structures of varying geometries were created and stabilized using hydrogels and cell-cell adhesion methods. Control of chemical microenvironments was achieved by the temporal release of specific factors from polymer microparticles positioned within these constructs. Complex co-culture micro-environmental analogues were also generated to reproduce structures found within adult stem cell niches. The application of holographic optical tweezers-based micromanipulation will enable novel insights into biological microenvironments by allowing researchers to form complex architectures with sub-micron precision of cells, matrices and molecules.

SUBMITTER: Kirkham GR 

PROVIDER: S-EPMC4341216 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Precision assembly of complex cellular microenvironments using holographic optical tweezers.

Kirkham Glen R GR   Britchford Emily E   Upton Thomas T   Ware James J   Gibson Graham M GM   Devaud Yannick Y   Ehrbar Martin M   Padgett Miles M   Allen Stephanie S   Buttery Lee D LD   Shakesheff Kevin K  

Scientific reports 20150226


The accurate study of cellular microenvironments is limited by the lack of technologies that can manipulate cells in 3D at a sufficiently small length scale. The ability to build and manipulate multicellular microscopic structures will facilitate a more detailed understanding of cellular function in fields such as developmental and stem cell biology. We present a holographic optical tweezers based technology to accurately generate bespoke cellular micro-architectures. Using embryonic stem cells,  ...[more]

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