Dysfunction in the ?II spectrin-dependent cytoskeleton underlies human arrhythmia.

ABSTRACT: The cardiac cytoskeleton plays key roles in maintaining myocyte structural integrity in health and disease. In fact, human mutations in cardiac cytoskeletal elements are tightly linked to cardiac pathologies, including myopathies, aortopathies, and dystrophies. Conversely, the link between cytoskeletal protein dysfunction and cardiac electric activity is not well understood and often overlooked in the cardiac arrhythmia field.Here, we uncover a new mechanism for the regulation of cardiac membrane excitability. We report that ?II spectrin, an actin-associated molecule, is essential for the posttranslational targeting and localization of critical membrane proteins in heart. ?II spectrin recruits ankyrin-B to the cardiac dyad, and a novel human mutation in the ankyrin-B gene disrupts the ankyrin-B/?II spectrin interaction, leading to severe human arrhythmia phenotypes. Mice lacking cardiac ?II spectrin display lethal arrhythmias, aberrant electric and calcium handling phenotypes, and abnormal expression/localization of cardiac membrane proteins. Mechanistically, ?II spectrin regulates the localization of cytoskeletal and plasma membrane/sarcoplasmic reticulum protein complexes, including the Na/Ca exchanger, ryanodine receptor 2, ankyrin-B, actin, and ?II spectrin. Finally, we observe accelerated heart failure phenotypes in ?II spectrin-deficient mice.Our findings identify ?II spectrin as critical for normal myocyte electric activity, link this molecule to human disease, and provide new insight into the mechanisms underlying cardiac myocyte biology.

SUBMITTER: Smith SA 

PROVIDER: S-EPMC4342332 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature