Ontology highlight
ABSTRACT:
SUBMITTER: Fortier S
PROVIDER: S-EPMC4343140 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Fortier Simon S MacRae Tara T Bilodeau Mélanie M Sargeant Tobias T Sauvageau Guy G
Proceedings of the National Academy of Sciences of the United States of America 20150202 7
In a functional genomics screen of mouse embryonic stem cells (ESCs) with nested hemizygous chromosomal deletions, we reveal that ribosomal protein (RP) genes are the most significant haploinsufficient determinants for embryoid body (EB) formation. Hemizygocity for three RP genes (Rps5, Rps14, or Rps28), distinguished by the proximity of their corresponding protein to the ribosome's mRNA exit site, is associated with the most profound phenotype. This EB phenotype was fully rescued by BAC or cDNA ...[more]