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Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.

ABSTRACT: We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

SUBMITTER: Ding Y

PROVIDER: S-EPMC4344385 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.

Ding Yidan Y Li Jiao J Enterina Jhon Ralph JR Shen Yi Y Zhang Issan I Tewson Paul H PH Mo Gary C H GC Zhang Jin J Quinn Anne Marie AM Hughes Thomas E TE Maysinger Dusica D Alford Spencer C SC Zhang Yan Y Campbell Robert E RE

Nature methods 20150126 3

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities. ...[more]

PMID: 25622108

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