Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression.

ABSTRACT:

Background

RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than one gene at a time. Here, we describe a glass-slide based miniaturized RNA dot blot (RNA array) procedure for rapid and parallel gene expression analysis using fluorescently labeled probes.

Results

RNA arrays were prepared by simple manual spotting of RNA onto amino-silane coated microarray glass slides, and used for two-color fluorescent hybridization with specific probes labeled with Cy3 and 18S ribosomal RNA house-keeping gene probe labeled with Cy5 fluorescent dyes. After hybridization, arrays were scanned on a fluorescent

SUBMITTER: Yadetie F 

PROVIDER: S-EPMC434516 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature