Characterizing protein modifications by reactive metabolites using magnetic bead bioreactors and LC-MS/MS.
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ABSTRACT:
SUBMITTER: Li D
PROVIDER: S-EPMC4348200 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Characterizing protein modifications by reactive metabolites using magnetic bead bioreactors and LC-MS/MS.
Li Dandan D Fu You-Jun YJ Rusling James F JF
Chemical communications (Cambridge, England) 20150301 22
We report here label-free metabolite-protein adduct detection and identification employing magnetic beads coated with metabolic enzymes as bioreactors to generate metabolites and possible metabolite-protein adducts for analysis by liquid chromatography-tandem mass spectrometry. ...[more]
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