Cloning and expression analysis of prohibitin mRNA in canine mammary tumors.
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ABSTRACT:
SUBMITTER: Matsuyama S
PROVIDER: S-EPMC4349545 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
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Cloning and expression analysis of prohibitin mRNA in canine mammary tumors.
Matsuyama Satoshi S Nakano Yuko Y Nakamura Mieko M Yamamoto Ryohei R Shimada Terumasa T Ohashi Fumihito F Kubo Kihei K
The Journal of veterinary medical science 20141014 1
Prohibitin is an antiproliferative protein that is a product of a putative tumor suppressor gene. However, there is little information on prohibitins in companion animals. In this study, we cloned canine prohibitin mRNA using RT-PCR and 3'-RACE (Rapid Amplification of cDNA Ends). The sequence was well conserved compared with those of other mammals, including human. The deduced amino acid sequence translated from the open reading frame completely corresponded to the human sequence. Canine prohibi ...[more]
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