Ontology highlight
ABSTRACT:
SUBMITTER: Albrecht E
PROVIDER: S-EPMC4352853 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Scientific reports 20150309
The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non ...[more]