Specific ion effects on macromolecular interactions in Escherichia coli extracts.
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ABSTRACT:
SUBMITTER: Kyne C
PROVIDER: S-EPMC4353357 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Specific ion effects on macromolecular interactions in Escherichia coli extracts.
Kyne Ciara C Ruhle Brian B Gautier Virginie W VW Crowley Peter B PB
Protein science : a publication of the Protein Society 20141230 3
Protein characterization in situ remains a major challenge for protein science. Here, the interactions of ΔTat-GB1 in Escherichia coli cell extracts were investigated by NMR spectroscopy and size exclusion chromatography (SEC). ΔTat-GB1 was found to participate in high molecular weight complexes that remain intact at physiologically-relevant ionic strength. This observation helps to explain why ΔTat-GB1 was not detected by in-cell NMR spectroscopy. Extracts pre-treated with RNase A had a differe ...[more]
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