TRAP-seq defines markers for novel populations of hypothalamic and brainstem LepRb neurons.

ABSTRACT:

Objective

Leptin acts via its receptor (LepRb) on multiple subpopulations of LepRb neurons in the brain, each of which controls specific aspects of energy balance. Despite the importance of LepRb-containing neurons, the transcriptome and molecular identity of many LepRb subpopulations remain undefined due to the difficulty of studying the small fraction of total cells represented by LepRb neurons in heterogeneous brain regions. Here we sought to examine the transcriptome of LepRb neurons directly and identify markers for functionally relevant LepRb subsets.

Methods

We isolated mRNA from mouse hypothalamic and brainstem LepRb cells by Translating Ribosome Affinity Purification (TRAP) and analyzed it by RNA-seq (TRAP-seq).

Results

TRAP mRNA from LepRb cells was enriched

SUBMITTER: Allison MB 

PROVIDER: S-EPMC4354921 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature