Ontology highlight
ABSTRACT:
SUBMITTER: Gardner BM
PROVIDER: S-EPMC4355278 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Gardner Brooke M BM Chowdhury Saikat S Lander Gabriel C GC Martin Andreas A
Journal of molecular biology 20150207 6 Pt B
Pex1 and Pex6 are Type-2 AAA+ ATPases required for the de novo biogenesis of peroxisomes. Mutations in Pex1 and Pex6 account for the majority of the most severe forms of peroxisome biogenesis disorders in humans. Here, we show that the ATP-dependent complex of Pex1 and Pex6 from Saccharomyces cerevisiae is a heterohexamer with alternating subunits. Within the Pex1/Pex6 complex, only the D2 ATPase ring hydrolyzes ATP, while nucleotide binding in the D1 ring promotes complex assembly. ATP hydrolys ...[more]