Sumoylation of CCAAT/enhancer-binding protein ? is implicated in hematopoietic stem/progenitor cell development through regulating runx1 in zebrafish.

ABSTRACT: The small ubiquitin-related modifier (SUMO) participates in various cellular processes, including maintenance of genome integrity, nuclear transport, transcription and signal transduction. However, the biological function of sumoylation in hematopoiesis has not been fully explored. We show here that definitive hematopoietic stem/progenitor cells (HSPCs) are depleted in SUMO-deficient zebrafish embryos. Impairment of sumoylation attenuates HSPC generation and proliferation. The hyposumoylation triggered HSPC defects are CCAAT/enhancer-binding protein ? (C/ebp?) dependent. Critically, a SUMO-C/ebp? fusion rescues the defective hematopoiesis in SUMO-deficient embryos, at least in part through restored runx1 expression. While C/ebp?-dependent transcription is involved in myeloid differentiation, our studies here reveal that C/ebp? sumoylation is essential for HSPC development during definitive hematopoiesis.

SUBMITTER: Yuan H 

PROVIDER: S-EPMC4355724 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature