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Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.

ABSTRACT: Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.

SUBMITTER: Stauber M

PROVIDER: S-EPMC4356360 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.

Stauber Mark M Jakoncic Jean J Berger Jacob J Karp Jerome M JM Axelbaum Ariel A Sastow Dahniel D Buldyrev Sergey V SV Hrnjez Bruce J BJ Asherie Neer N

Acta crystallographica. Section D, Biological crystallography 20150226 Pt 3

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the ob ...[more]

PMID: 25760593

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