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Analysis of pollutant-induced changes in mitochondrial DNA methylation.


ABSTRACT: There is increasing evidence that exposure to air pollutants is associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome are also applicable to mitochondrial epigenetic studies. In this chapter, we describe a protocol for the isolation of mitochondrial DNA from peripheral blood samples and the analysis of 5-methylcytosine content by bisulfite pyrosequencing.

SUBMITTER: Byun HM 

PROVIDER: S-EPMC4356890 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Analysis of pollutant-induced changes in mitochondrial DNA methylation.

Byun Hyang-Min HM   Barrow Timothy M TM  

Methods in molecular biology (Clifton, N.J.) 20150101


There is increasing evidence that exposure to air pollutants is associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome  ...[more]

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