Ontology highlight
ABSTRACT:
SUBMITTER: Huang T
PROVIDER: S-EPMC4359002 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Huang Tinghua T Yang Jun J Liu Guopin G Jin Wei W Liu Zhi Z Zhao Shuhong S Yao Min M
PloS one 20150313 3
The robust and reliable detection of small microRNAs (miRNAs) is important to understand the functional significance of miRNAs. Several methods can be used to quantify miRNAs. Selectively quantifying mature miRNAs among miRNA precursors, pri-miRNAs, and other miRNA-like sequences is challenging because of the short length of miRNAs. In this study, we developed a two-step miRNA quantification system based on pincer probe capture and real-time PCR amplification. The performance of the method was t ...[more]