Unknown

Dataset Information

0

Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry.


ABSTRACT: Entry into mitosis is accompanied by profound changes in cortical actomyosin organization. Here, we delineate a pathway downstream of the RhoGEF Pbl/Ect2 that directs this process in a model epithelium. Our data suggest that the release of Pbl/Ect2 from the nucleus at mitotic entry drives Rho-dependent activation of Myosin-II and, in parallel, induces a switch from Arp2/3 to Diaphanous-mediated cortical actin nucleation that depends on Cdc42, aPKC, and Par6. At the same time, the mitotic relocalization of these apical protein complexes to more lateral cell surfaces enables Cdc42/aPKC/Par6 to take on a mitosis-specific function-aiding the assembly of a relatively isotropic metaphase cortex. Together, these data reveal how the repolarization and remodeling of the actomyosin cortex are coordinated upon entry into mitosis to provide cells with the isotropic and rigid form they need to undergo faithful chromosome segregation and division in a crowded tissue environment.

SUBMITTER: Rosa A 

PROVIDER: S-EPMC4359025 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry.

Rosa André A   Vlassaks Evi E   Pichaud Franck F   Baum Buzz B  

Developmental cell 20150219 5


Entry into mitosis is accompanied by profound changes in cortical actomyosin organization. Here, we delineate a pathway downstream of the RhoGEF Pbl/Ect2 that directs this process in a model epithelium. Our data suggest that the release of Pbl/Ect2 from the nucleus at mitotic entry drives Rho-dependent activation of Myosin-II and, in parallel, induces a switch from Arp2/3 to Diaphanous-mediated cortical actin nucleation that depends on Cdc42, aPKC, and Par6. At the same time, the mitotic relocal  ...[more]

Similar Datasets

| S-EPMC3742451 | biostudies-literature
| S-EPMC3763371 | biostudies-literature
| S-EPMC7178822 | biostudies-literature
2020-01-29 | GSE139856 | GEO
2020-01-30 | GSE139846 | GEO
| S-EPMC7948514 | biostudies-literature
2020-01-30 | GSE139845 | GEO
2020-01-30 | GSE130558 | GEO
| PRJNA587151 | ENA
| S-EPMC7521848 | biostudies-literature