Ontology highlight
ABSTRACT:
SUBMITTER: Boudreaux CE
PROVIDER: S-EPMC4359669 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Boudreaux Crystal E CE Kelly Deborah F DF McDonald Sarah M SM
Virology 20150128
Rotaviruses (RVs) replicate their segmented, double-stranded RNA genomes in tandem with early virion assembly. In this study, we sought to gain insight into the ultrastructure of RV assembly-replication intermediates (RIs) using transmission electron microscopy (EM). Specifically, we examined a replicase-competent, subcellular fraction that contains all known RV RIs. Three never-before-seen complexes were visualized in this fraction. Using in vitro reconstitution, we showed that ~15-nm doughnut- ...[more]