Unknown

Dataset Information

0

Biotransformation of the mycotoxin deoxynivalenol in fusarium resistant and susceptible near isogenic wheat lines.


ABSTRACT: In this study, a total of nine different biotransformation products of the Fusarium mycotoxin deoxynivalenol (DON) formed in wheat during detoxification of the toxin are characterized by liquid chromatography-high resolution mass spectrometry (LC-HRMS). The detected metabolites suggest that DON is conjugated to endogenous metabolites via two major metabolism routes, namely 1) glucosylation (DON-3-glucoside, DON-di-hexoside, 15-acetyl-DON-3-glucoside, DON-malonylglucoside) and 2) glutathione conjugation (DON-S-glutathione, "DON-2H"-S-glutathione, DON-S-cysteinyl-glycine and DON-S-cysteine). Furthermore, conjugation of DON to a putative sugar alcohol (hexitol) was found. A molar mass balance for the cultivar 'Remus' treated with 1 mg DON revealed that under the test conditions approximately 15% of the added DON were transformed into DON-3-glucoside and another 19% were transformed to the remaining eight biotransformation products or irreversibly bound to the plant matrix. Additionally, metabolite abundance was monitored as a function of time for each DON derivative and was established for six DON treated wheat lines (1 mg/ear) differing in resistance quantitative trait loci (QTL) Fhb1 and/or Qfhs.ifa-5A. All cultivars carrying QTL Fhb1 showed similar metabolism kinetics: Formation of DON-Glc was faster, while DON-GSH production was less efficient compared to cultivars which lacked the resistance QTL Fhb1. Moreover, all wheat lines harboring Fhb1 showed significantly elevated D3G/DON abundance ratios.

SUBMITTER: Kluger B 

PROVIDER: S-EPMC4361057 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Biotransformation of the mycotoxin deoxynivalenol in fusarium resistant and susceptible near isogenic wheat lines.

Kluger Bernhard B   Bueschl Christoph C   Lemmens Marc M   Michlmayr Herbert H   Malachova Alexandra A   Koutnik Andrea A   Maloku Imer I   Berthiller Franz F   Adam Gerhard G   Krska Rudolf R   Schuhmacher Rainer R  

PloS one 20150316 3


In this study, a total of nine different biotransformation products of the Fusarium mycotoxin deoxynivalenol (DON) formed in wheat during detoxification of the toxin are characterized by liquid chromatography-high resolution mass spectrometry (LC-HRMS). The detected metabolites suggest that DON is conjugated to endogenous metabolites via two major metabolism routes, namely 1) glucosylation (DON-3-glucoside, DON-di-hexoside, 15-acetyl-DON-3-glucoside, DON-malonylglucoside) and 2) glutathione conj  ...[more]

Similar Datasets

| S-EPMC2920755 | biostudies-literature
| S-EPMC4769521 | biostudies-literature
| S-EPMC6640518 | biostudies-other
| S-EPMC5434243 | biostudies-literature
2018-02-28 | E-MTAB-6383 | biostudies-arrayexpress
| S-EPMC9832699 | biostudies-literature
| S-EPMC10220118 | biostudies-literature
| S-EPMC5129171 | biostudies-literature
2015-06-03 | GSE54556 | GEO
| S-EPMC4700149 | biostudies-literature