Ontology highlight
ABSTRACT:
SUBMITTER: Zhan X
PROVIDER: S-EPMC4361079 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Zhan Xuanzhi X Kook Seunghyi S Kaoud Tamer S TS Dalby Kevin N KN Gurevich Eugenia V EV Gurevich Vsevolod V VV
Current protocols in pharmacology 20150302
Only one out of four mammalian arrestin subtypes, arrestin-3, facilitates the activation of JNK family kinases. Here we describe two different protocols used for elucidating the mechanisms involved. One is based on reconstitution of signaling modules from purified proteins: arrestin-3, MKK4, MKK7, JNK1, JNK2, and JNK3. The main advantage of this method is that it unambiguously establishes which effects are direct because only intended purified proteins are present in these assays. The key drawba ...[more]