Ontology highlight
ABSTRACT:
SUBMITTER: Dow BA
PROVIDER: S-EPMC4363176 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Dow Brian A BA Tatulian Suren A SA Davidson Victor L VL
Protein expression and purification 20150105
Periplasmic localization of recombinant proteins offers advantages over cytoplasmic protein expression. In this study signal sequence of amicyanin, which is encoded by the mauC gene of Paracoccus denitrificans, was used to express the light chain variable domain of the human κIO8/O18 germline antibody in the periplasm of Escherichiacoli. The expressed protein was purified in good yield (70mg/L of culture) in one step from the periplasmic fraction by affinity chromatography using an engineered he ...[more]