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Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition.

ABSTRACT: This study evaluates the effect of low oxygen conditions (5 Vs 20%) on buffalo embryo development. Expression patterns of key glucose metabolism genes (HK, PFK, LDH, PDH, G6PDH and Glut1) were assessed in buffalo oocytes and embryos cultured at 5 and 20% oxygen and correlated with development rate.Maturation rate was observed by determining MII stages by Aceto-orcein method and blastocyst formation was observed at 7 day post insemination (dpi). Expression levels of genes were determined by real time PCR in oocytes / embryos at 5 and 20% O2.Oocyte maturation and blastocyst formation rates were significantly higher at 5% O2 as compared to 20% O2 (P?

SUBMITTER: Kumar P

PROVIDER: S-EPMC4363242 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition.

Kumar Parveen P Verma Arpana A Kumar Manish M De Sachinandan S Kumar Rakesh R Datta Tirtha Kumar TK

Journal of assisted reproduction and genetics 20150113 3

<h4>Purpose</h4>This study evaluates the effect of low oxygen conditions (5 Vs 20%) on buffalo embryo development. Expression patterns of key glucose metabolism genes (HK, PFK, LDH, PDH, G6PDH and Glut1) were assessed in buffalo oocytes and embryos cultured at 5 and 20% oxygen and correlated with development rate.<h4>Methods</h4>Maturation rate was observed by determining MII stages by Aceto-orcein method and blastocyst formation was observed at 7 day post insemination (dpi). Expression levels o ...[more]

PMID: 25578537

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