Effects of urotensin-II on cytokines in early acute liver failure in mice.

ABSTRACT: To investigate urotensin-II (UII) and its effects on tumor necrosis factor (TNF)-? and interleukin (IL)-1? in early acute liver failure (ALF).We investigated the time-dependent alteration in UII levels and its effects on TNF-? and IL-1? in liver and blood in the early stage of lipopolysaccharide/D-galactosamine-induced ALF.After lipopolysaccharide/D-galactosamine challenge, UII rose very rapidly and reached a maximal level 0.5 h, and the level remained significantly elevated after 2 h (P < 0.05). Six hours after challenge, UII began to degrade, but remained higher than at 0 h (P < 0.05). Pretreatment with urantide, an inhibitor of the UII receptor, suppressed the degree of UII increase in liver and blood at 6 h after challenge (P < 0.05 vs paired controls). In addition, liver and blood TNF-? increased from 1 to 6 h, and reached a peak at 1 and 2 h, respectively; however, IL-1? did not rise until 6 h after challenge. Urantide pretreatment inhibited the degree of TNF-? and IL-1? increase following downregulation of UII post-challenge (all P < 0.05).UII plays a role in the pathogenesis and priming of ALF by triggering an inflammatory cascade and driving the early release of cytokines in mice.

SUBMITTER: Liu LM 

PROVIDER: S-EPMC4363753 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature