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Separation of folinic acid diastereomers in capillary electrophoresis using a new cationic ?-cyclodextrin derivative.


ABSTRACT: A method for the separation of folinic acid diastereomers by capillary electrophoresis in chiral separation media was developed. Aiming to achieve a good separation of the anionic analytes, a newly synthesized cationic ?-cyclodextrin derivative, mono-6-deoxy-6-piperdine-?-cyclodextrin, was applied as the chiral selector. The effect of background electrolyte pH, the concentration of the cyclodextrin additive, and organic modifier on the separation was investigated. A good separation of folinic acid diastereomers was obtained with 30 mmol/L phosphate buffer at pH 6.50 containing 6.0 mmol/L of mono-6-deoxy-6-piperdine-?-cyclodextrin in 10% acetonitrile. Based on the capillary electrophoresis data, the binding constants of each diastereomer with mono-6-deoxy-6-piperdine-?-cyclodextrin were determined. Moreover, a computational modeling study, using the semi-empirical PM3 method, was used to discuss the possible mechanism of separation of folinic acid with mono-6-deoxy-6-piperdine-?-cyclodextrin.

SUBMITTER: Yu J 

PROVIDER: S-EPMC4364531 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Separation of folinic acid diastereomers in capillary electrophoresis using a new cationic β-cyclodextrin derivative.

Yu Jia J   Liang Xinlei X   Wang Zhaokun Z   Guo Xin X   Sun Tiemin T   Guo Xingjie X  

PloS one 20150317 3


A method for the separation of folinic acid diastereomers by capillary electrophoresis in chiral separation media was developed. Aiming to achieve a good separation of the anionic analytes, a newly synthesized cationic β-cyclodextrin derivative, mono-6-deoxy-6-piperdine-β-cyclodextrin, was applied as the chiral selector. The effect of background electrolyte pH, the concentration of the cyclodextrin additive, and organic modifier on the separation was investigated. A good separation of folinic ac  ...[more]

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