Unknown

Dataset Information

0

Genetic analysis of the CDI pathway from Burkholderia pseudomallei 1026b.


ABSTRACT: Contact-dependent growth inhibition (CDI) is a mode of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion systems. CdiA binds to receptors on susceptible target bacteria, then delivers a toxin domain derived from its C-terminus. Studies with Escherichia coli suggest the existence of multiple CDI growth-inhibition pathways, whereby different systems exploit distinct target-cell proteins to deliver and activate toxins. Here, we explore the CDI pathway in Burkholderia using the CDIIIBp1026b system encoded on chromosome II of Burkholderia pseudomallei 1026b as a model. We took a genetic approach and selected Burkholderia thailandensis E264 mutants that are resistant to growth inhibition by CDIIIBp1026b. We identified mutations in three genes, BTH_I0359, BTH_II0599, and BTH_I0986, each of which confers resistance to CDIIIBp1026b. BTH_I0359 encodes a small peptide of unknown function, whereas BTH_II0599 encodes a predicted inner membrane transport protein of the major facilitator superfamily. The inner membrane localization of BTH_II0599 suggests that it may facilitate translocation of CdiA-CTIIBp1026b toxin from the periplasm into the cytoplasm of target cells. BTH_I0986 encodes a putative transglycosylase involved in lipopolysaccharide (LPS) synthesis. ?BTH_I0986 mutants have altered LPS structure and do not interact with CDI? inhibitor cells to the same extent as BTH_I0986? cells, suggesting that LPS could function as a receptor for CdiAIIBp1026b. Although ?BTH_I0359, ?BTH_II0599, and ?BTH_I0986 mutations confer resistance to CDIIIBp1026b, they provide no protection against the CDIE264 system deployed by B. thailandensis E264. Together, these findings demonstrate that CDI growth-inhibition pathways are distinct and can differ significantly even between closely related species.

SUBMITTER: Koskiniemi S 

PROVIDER: S-EPMC4364669 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genetic analysis of the CDI pathway from Burkholderia pseudomallei 1026b.

Koskiniemi Sanna S   Garza-Sánchez Fernando F   Edman Natasha N   Chaudhuri Swarnava S   Poole Stephen J SJ   Manoil Colin C   Hayes Christopher S CS   Low David A DA  

PloS one 20150318 3


Contact-dependent growth inhibition (CDI) is a mode of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion systems. CdiA binds to receptors on susceptible target bacteria, then delivers a toxin domain derived from its C-terminus. Studies with Escherichia coli suggest the existence of multiple CDI growth-inhibition pathways, whereby different systems exploit distinct target-cell proteins to deliver and activate toxins. Here, we explore the CDI pathway in Burkhold  ...[more]

Similar Datasets

| S-EPMC3331888 | biostudies-literature
| S-EPMC8917201 | biostudies-literature
| S-EPMC4107055 | biostudies-literature
| S-EPMC2258562 | biostudies-literature
| S-EPMC1853089 | biostudies-other
| S-EPMC7998038 | biostudies-literature
| S-EPMC7920660 | biostudies-literature
2011-12-31 | GSE27558 | GEO
| S-EPMC2646673 | biostudies-literature
| S-EPMC4236580 | biostudies-literature