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Variola virus-specific diagnostic assays: characterization, sensitivity, and specificity.


ABSTRACT: A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.

SUBMITTER: Kondas AV 

PROVIDER: S-EPMC4365196 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Variola virus-specific diagnostic assays: characterization, sensitivity, and specificity.

Kondas Ashley V AV   Olson Victoria A VA   Li Yu Y   Abel Jason J   Laker Miriam M   Rose Laura L   Wilkins Kimberly K   Turner Jonathan J   Kline Richard R   Damon Inger K IK  

Journal of clinical microbiology 20150211 4


A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified. ...[more]

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