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Development of a peptide nucleic acid-based multiprobe real-time PCR method targeting the hsp65 gene for differentiation among Mycobacterium abscessus strains.


ABSTRACT: Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense).

SUBMITTER: Kim K 

PROVIDER: S-EPMC4365251 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Development of a peptide nucleic acid-based multiprobe real-time PCR method targeting the hsp65 gene for differentiation among Mycobacterium abscessus strains.

Kim Kijeong K   Kim Byoung-Jun BJ   Shim Tae Sun TS   Hong Seok-Hyun SH   Kook Yoon-Hoh YH   Kim Bum-Joon BJ  

Journal of clinical microbiology 20150204 4


Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense). ...[more]

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