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A simple bioluminescent method for measuring D-amino acid oxidase activity.

ABSTRACT: D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

SUBMITTER: Bailey TS

PROVIDER: S-EPMC4365669 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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A simple bioluminescent method for measuring D-amino acid oxidase activity.

Bailey T Spencer TS Donor Micah T MT Naughton Sean P SP Pluth Michael D MD

Chemical communications (Cambridge, England) 20150301 25

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin. ...[more]

PMID: 25408176

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