Ontology highlight
ABSTRACT:
SUBMITTER: Kowalsky CA
PROVIDER: S-EPMC4366243 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Kowalsky Caitlin A CA Klesmith Justin R JR Stapleton James A JA Kelly Vince V Reichkitzer Nolan N Whitehead Timothy A TA
PloS one 20150319 3
Comprehensive sequence-function mapping involves detailing the fitness contribution of every possible single mutation to a gene by comparing the abundance of each library variant before and after selection for the phenotype of interest. Deep sequencing of library DNA allows frequency reconstruction for tens of thousands of variants in a single experiment, yet short read lengths of current sequencers makes it challenging to probe genes encoding full-length proteins. Here we extend the scope of se ...[more]