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Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.

ABSTRACT: We evaluated in vivo innate immune responses in monocyte populations from 67 young (aged 21-30 years) and older (aged ?65 years) adults before and after influenza vaccination. CD14(+)CD16(+) inflammatory monocytes were induced after vaccination in both young and older adults. In classical CD14(+)CD16(-) and inflammatory monocytes, production of tumor necrosis factor ? and interleukin 6, as measured by intracellular staining, was strongly induced after vaccination. Cytokine production was strongly associated with influenza vaccine antibody response; the highest levels were found as late as day 28 after vaccination in young subjects and were substantially diminished in older subjects. Notably, levels of the anti-inflammatory cytokine interleukin 10 (IL-10) were markedly elevated in monocytes from older subjects before and after vaccination. In purified monocytes, we found age-associated elevation in phosphorylated signal transducer and activator of transcription-3, and decreased serine 359 phosphorylation of the negative IL-10 regulator dual-specificity phosphatase 1. These findings for the first time implicate dysregulated IL-10 production in impaired vaccine responses in older adults.

SUBMITTER: Mohanty S

PROVIDER: S-EPMC4366602 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.

Mohanty Subhasis S Joshi Samit R SR Ueda Ikuyo I Wilson Jean J Blevins Tamara P TP Siconolfi Barbara B Meng Hailong H Devine Lesley L Raddassi Khadir K Tsang Sui S Belshe Robert B RB Hafler David A DA Kaech Susan M SM Kleinstein Steven H SH Trentalange Mark M Allore Heather G HG Shaw Albert C AC

The Journal of infectious diseases 20141102 7

We evaluated in vivo innate immune responses in monocyte populations from 67 young (aged 21-30 years) and older (aged ≥65 years) adults before and after influenza vaccination. CD14(+)CD16(+) inflammatory monocytes were induced after vaccination in both young and older adults. In classical CD14(+)CD16(-) and inflammatory monocytes, production of tumor necrosis factor α and interleukin 6, as measured by intracellular staining, was strongly induced after vaccination. Cytokine production was strongl ...[more]

PMID: 25367297

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Dataset Information

Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.

Publications

Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.

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