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CIKS/DDX3X interaction controls the stability of the Zc3h12a mRNA induced by IL-17.


ABSTRACT: IL-17 is a proinflammatory cytokine that promotes the expression of different cytokines and chemokines via the induction of gene transcription and the posttranscriptional stabilization of mRNAs. In this study, we show that IL-17 increases the half-life of the Zc3h12a mRNA via interaction of the adaptor protein CIKS with the DEAD box protein DDX3X. IL-17 stimulation promotes the formation of a complex between CIKS and DDX3X, and this interaction requires the helicase domain of DDX3X but not its ATPase activity. DDX3X knockdown decreases the IL-17-induced stability of Zc3h12a without affecting the stability of other mRNAs. IKK?, TNFR-associated factor 2, and TNFR-associated factor 5 were also required to mediate the IL-17-induced Zc3h12a stabilization. DDX3X directly binds the Zc3h12a mRNA after IL-17 stimulation. Collectively, our findings define a novel, IL-17-dependent mechanism regulating the stabilization of a selected mRNA.

SUBMITTER: Somma D 

PROVIDER: S-EPMC4369453 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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CIKS/DDX3X interaction controls the stability of the Zc3h12a mRNA induced by IL-17.

Somma Domenico D   Mastrovito Paola P   Grieco Marianeve M   Lavorgna Alfonso A   Pignalosa Angelica A   Formisano Luigi L   Salzano Anna Maria AM   Scaloni Andrea A   Pacifico Francesco F   Siebenlist Ulrich U   Leonardi Antonio A  

Journal of immunology (Baltimore, Md. : 1950) 20150220 7


IL-17 is a proinflammatory cytokine that promotes the expression of different cytokines and chemokines via the induction of gene transcription and the posttranscriptional stabilization of mRNAs. In this study, we show that IL-17 increases the half-life of the Zc3h12a mRNA via interaction of the adaptor protein CIKS with the DEAD box protein DDX3X. IL-17 stimulation promotes the formation of a complex between CIKS and DDX3X, and this interaction requires the helicase domain of DDX3X but not its A  ...[more]

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