A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.
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ABSTRACT: We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
SUBMITTER: Bell L
PROVIDER: S-EPMC4375559 | biostudies-literature |
REPOSITORIES: biostudies-literature
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