Ontology highlight
ABSTRACT:
SUBMITTER: Wang K
PROVIDER: S-EPMC4377304 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Wang Ke K Wang Danzhu D Ji Kaili K Chen Weixuan W Zheng Yueqin Y Dai Chaofeng C Wang Binghe B
Organic & biomolecular chemistry 20150101 3
Post-synthesis DNA modification is a very useful method for DNA functionalization. This is achieved by using a modified NTP, which has a handle for further modifications, replacing the corresponding natural NTP in polymerase-catalyzed DNA synthesis. Subsequently, the handle can be used for further functionalization after PCR, preferably through a very fast reaction. Herein we describe polymerase-mediated incorporation of trans-cyclooctene modified thymidine triphosphate (TCO-TTP). Subsequently, ...[more]