Ontology highlight
ABSTRACT:
SUBMITTER: Chapman AR
PROVIDER: S-EPMC4378937 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
PloS one 20150330 3
Recently, Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) has been developed for whole genome amplification of an individual cell, relying on quasilinear instead of exponential amplification to achieve high coverage. Here we adapt MALBAC for single-cell transcriptome amplification, which gives consistently high detection efficiency, accuracy and reproducibility. With this newly developed technique, we successfully amplified and sequenced single cells from 3 germ layers from mo ...[more]