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Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

ABSTRACT: OBJECTIVE:We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge. MATERIAL AND METHODS:A total of 18 patients with allergic asthma (AA), 14 with allergic rhinitis (AR), and 10 healthy subjects (HS) underwent bronchial challenge with a specific allergen extract. Eosinophils from peripheral blood were isolated 24 h before as well as 7 and 24 h after bronchial allergen challenge. Chemotaxis, spontaneous ROS production in eosinophils, and apoptosis were analyzed by flow cytometry. Serum and induced sputum IL-5 levels were measured by ELISA; the cell count in sputum was analyzed by the May-Grünwald-Giemsa method. RESULTS:Before bronchial allergen challenge, peripheral blood eosinophil chemotaxis, spontaneous ROS production was enhanced and eosinophil apoptosis was reduced in the patients with AA as compared with AR patients and HS (P?

SUBMITTER: Lavinskiene S

PROVIDER: S-EPMC4379944 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

Lavinskiene Simona S Malakauskas Kestutis K Jeroch Jolanta J Hoppenot Deimante D Sakalauskas Raimundas R

Journal of inflammation (London, England) 20150329

<h4>Objective</h4>We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.<h4>Material and methods</h4>A total of 18 patients with allergic asthma (AA), 14 with allergic rhinitis (AR), and 10 healthy subjects (HS) underwent bronchial challenge with a specific allergen extract. Eosinophils from peripheral blood were isolated 24 h before as well as 7 an ...[more]

PMID: 25829869

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Project description:Previous in vitro and animal studies demonstrated that transcription factors p-STAT6 and PU.1 are required to induce interleukin (IL)-9 secretion by T helper (Th) 9 cells. It is believed that n factor-kappaB (NF-?B) plays a role in eosinophil survival. The importance of these transcription factors in the pathogenesis of allergic asthma (AA) in humans is poorly understood. We evaluated p-STAT6 and PU.1 expression in peripheral blood Th9 cells and NF-?B expression in eosinophils during late-phase airway inflammation in AA patients.Nineteen adults with AA and 14 adult healthy individuals (HI) were examined. Peripheral blood collected 24 h before (baseline) and 24 h after bronchial allergen challenge. CD4(+) cells and eosinophils were isolated by high-density gradient centrifugation and magnetic separation. The percentage of Th9 cells and apoptotic eosinophils was estimated by flow cytometry. p-STAT6 and PU.1 expression was expressed as mean fluorescence intensity (MFI) in Th9 cells. NF-?B levels were expressed as MFI in peripheral blood eosinophils. Serum IL-9 and IL-5 levels were determined by enzyme-linked immunosorbent assay.At baseline, MFI of p-STAT6 and PU.1 in peripheral blood Th9 cells and MFI of NF-?B in eosinophils and, serum IL-5 and IL-9 levels were greater in AA patients (P < 0.05). Decreased eosinophil apoptosis was seen in the AA group compared with HI (P < 0.05). MFI of p-STAT6, PU.1, and NF-?B and serum levels of IL-5 and IL-9 were increased in the AA group 24 h after challenge compared with baseline (P < 0.05). In the AA group, a correlation between serum IL-9 and Th9 cells (r = 0.7, P = 0.001) and MFI of PU.1 (r = 0.6, P = 0.01) 24 h after bronchial allergen challenge was observed. A correlation between Th9 cells and MFI of p-STAT6 (r = 0.45, P = 0.03) as well as MFI of PU.1 (r = 0.5, P = 0.02) 24 h after challenge was only observed in AA patients. A correlation between the MFI of NF-?B and eosinophil apoptosis was observed in AA patients 24 h before (r =?- .46, P = 0.02) and after (r = -0.5, P = 0.02) challenge.p-STAT6 and PU.1 may be associated with Th9 cells and IL-9 production, whereas NF-?B and IL-5 may be associated with reduced eosinophil apoptosis in allergen-induced late-phase airway inflammation.ClinicalTrials.gov NCT02214303.

Dataset Information

Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

Publications

Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

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