Ontology highlight
ABSTRACT:
SUBMITTER: Madsen JG
PROVIDER: S-EPMC4381047 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Madsen Jesper Grud Skat JG Schmidt Søren Fisker SF Larsen Bjørk Ditlev BD Loft Anne A Nielsen Ronni R Mandrup Susanne S
Nucleic acids research 20150106 6
RNA-seq is a sensitive and accurate technique to compare steady-state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se, other technologies are needed to more precisely determine acute transcriptional responses. Here, we have developed an easy, sensitive and accurate novel computational method, IRNA-SEQ: , for genome-wide assessment of transcriptional activity based on analysis of intron coverage from total RNA-seq data ...[more]