Ontology highlight
ABSTRACT:
SUBMITTER: Gautam A
PROVIDER: S-EPMC4381068 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Gautam Amit A Grainger Richard J RJ Vilardell J J Barrass J David JD Beggs Jean D JD
Nucleic acids research 20150303 6
Pre-mRNA splicing involves two transesterification steps catalyzed by the spliceosome. How RNA substrates are positioned in each step and the molecular rearrangements involved, remain obscure. Here, we show that mutations in PRP16, PRP8, SNU114 and the U5 snRNA that affect this process interact genetically with CWC21, that encodes the yeast orthologue of the human SR protein, SRm300/SRRM2. Our microarray analysis shows changes in 3' splice site selection at elevated temperature in a subset of in ...[more]