Unknown

Dataset Information

0

CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells.


ABSTRACT: Metastasis is considered a dynamic process in tumor development that is related to abnormal migration and invasion. Tumor cells can move as individual cells in two interconvertible modes: mesenchymal-type and amoeboid. Previously, we reported that the interaction between CD147 and Annexin II can inhibit the amoeboid movement in hepatocellular carcinoma (HCC) cells. However, the mechanism of CD147 involved in mesenchymal movement is still unclear. Notably, our results show overexpression of CD147 led to mesenchymal-type movement in HCC cells. Evidence indicated that the mesenchymal-type cell movement induced by CD147 was Src dependent, as observed by confocal microscopy and Rac1 activity assay. The phosphorylation of Src (pY416-Src) can be up-regulated by CD147, and this regulation is mediated by focal adhesion kinase (FAK). Next, we identified DOCK8 as a GEF for Rac1, a key molecule driving mesenchymal-type movement. We also found that Src promotes STAT3 phosphorylation and STAT3 facilitates DOCK8 transcription, thus enhancing DOCK8 expression and Rac1 activation. This study provides a novel mechanism of CD147 regulating mesenchymal-type movement in HCC cells.

SUBMITTER: Wang SJ 

PROVIDER: S-EPMC4381592 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells.

Wang Shi-Jie SJ   Cui Hong-Yong HY   Liu Yan-Mei YM   Zhao Pu P   Zhang Yang Y   Fu Zhi-Guang ZG   Chen Zhi-Nan ZN   Jiang Jian-Li JL  

Oncotarget 20150101 1


Metastasis is considered a dynamic process in tumor development that is related to abnormal migration and invasion. Tumor cells can move as individual cells in two interconvertible modes: mesenchymal-type and amoeboid. Previously, we reported that the interaction between CD147 and Annexin II can inhibit the amoeboid movement in hepatocellular carcinoma (HCC) cells. However, the mechanism of CD147 involved in mesenchymal movement is still unclear. Notably, our results show overexpression of CD147  ...[more]

Similar Datasets

| S-EPMC6115913 | biostudies-literature
| S-EPMC7525663 | biostudies-literature
| S-EPMC7038622 | biostudies-literature
| S-EPMC7391525 | biostudies-literature
2014-08-08 | E-GEOD-60194 | biostudies-arrayexpress
| S-EPMC4741493 | biostudies-literature
| S-EPMC8571373 | biostudies-literature
2020-02-21 | GSE145636 | GEO
| S-EPMC4891050 | biostudies-literature
2014-08-08 | GSE60194 | GEO