Unknown

Dataset Information

0

Development and evaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes.


ABSTRACT: BACKGROUND AND OBJECTIVES:We developed and evaluated the utility of a quadruplex Taqman real-time PCR assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. MATERIALS AND METHODS:The specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were identified and subsequently genotyped using a Quadruplex Taqman real-time PCR assay and melting curve analysis. Representative Quadruplex Taqman real-time PCR amplification curve were obtained for Enterococcus faecium, Enterococcus faecalis, vanA-containing E. faecium, vanB-containing E. faecalis. RESULTS:Phenotypic and genotypic analysis of the isolates gave same results for 82 enterococcal isolates, while in 5 isolates, they were inconsistent. We had three mixed strains, which were detected by the TaqMan real-time PCR assay and could not be identified correctly using phenotypic methods. CONCLUSION:Vancomycin resistant enterococci (VRE) genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using TaqMan real-time multiplex real-time PCR assay.

SUBMITTER: Naserpour Farivar T 

PROVIDER: S-EPMC4385574 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Development and evaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes.

Naserpour Farivar Taghi T   Najafipour Reza R   Johari Pouran P   Aslanimehr Masoumeh M   Peymani Amir A   Jahani Hashemi Hoasan H   Mirzaui Baman B  

Iranian journal of microbiology 20141001 5


<h4>Background and objectives</h4>We developed and evaluated the utility of a quadruplex Taqman real-time PCR assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci.<h4>Materials and methods</h4>The specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were identified and subsequently genotyped using a Quadruplex Taqman real-time PCR assay and me  ...[more]

Similar Datasets

| S-EPMC4512060 | biostudies-literature
| S-EPMC2546774 | biostudies-literature
| S-EPMC5766742 | biostudies-literature
| S-EPMC188027 | biostudies-other
| S-EPMC85849 | biostudies-literature
2020-11-25 | GSE115009 | GEO
| S-EPMC9489580 | biostudies-literature
| S-EPMC5916999 | biostudies-literature
| S-EPMC521886 | biostudies-literature
| S-EPMC4473229 | biostudies-literature