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Whole-genome expression profiling of Thermotoga maritima in response to growth on sugars in a chemostat.

ABSTRACT: To provide data necessary to study catabolite-linked transcriptional networks in Thermotoga maritima, we used full-genome DNA microarray analysis of global transcriptional responses to growth on glucose, lactose, and maltose in a chemostat. A much larger number of genes changed expression in cells grown on lactose than on maltose, each relative to genes expressed in cells grown on glucose. Genes encoding putative oligopeptide transporters were often coregulated with adjacent glycosidase-encoding genes. Genes encoding enzymes catalyzing NADH oxidation were up-regulated on both lactose and maltose. Genes involved in iron and sulfur metabolism were differentially expressed in response to lactose. These data help define the sets of coregulated genes and suggest possible functions for their encoded products.

SUBMITTER: Nguyen TN

PROVIDER: S-EPMC438634 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

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Whole-genome expression profiling of Thermotoga maritima in response to growth on sugars in a chemostat.

Nguyen Tu N TN Ejaz Arvin D AD Brancieri Mark A MA Mikula Amy M AM Nelson Karen E KE Gill Steven R SR Noll Kenneth M KM

Journal of bacteriology 20040701 14

To provide data necessary to study catabolite-linked transcriptional networks in Thermotoga maritima, we used full-genome DNA microarray analysis of global transcriptional responses to growth on glucose, lactose, and maltose in a chemostat. A much larger number of genes changed expression in cells grown on lactose than on maltose, each relative to genes expressed in cells grown on glucose. Genes encoding putative oligopeptide transporters were often coregulated with adjacent glycosidase-encoding ...[more]

PMID: 15231816

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Several archaeal homologs of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars.

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| S-EPMC1392961 | biostudies-literature

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Thermotoga maritima wild type minimal media expression analysis

Project description:An eight chip study using total RNA recovered from separate wild-type cultures of Thermotoga maritima at mid-log with 3 different minimal sugar media.

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Expression, purification, and characterization of Thermotoga maritima membrane proteins for structure determination.

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