Herpes simplex viral-vector design for efficient transduction of nonneuronal cells without cytotoxicity.
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ABSTRACT:
SUBMITTER: Miyagawa Y
PROVIDER: S-EPMC4386379 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Herpes simplex viral-vector design for efficient transduction of nonneuronal cells without cytotoxicity.
Miyagawa Yoshitaka Y Marino Pietro P Verlengia Gianluca G Uchida Hiroaki H Goins William F WF Yokota Shinichiro S Geller David A DA Yoshida Osamu O Mester Joseph J Cohen Justus B JB Glorioso Joseph C JC
Proceedings of the National Academy of Sciences of the United States of America 20150316 13
The design of highly defective herpes simplex virus (HSV) vectors for transgene expression in nonneuronal cells in the absence of toxic viral-gene activity has been elusive. Here, we report that elements of the latency locus protect a nonviral promoter against silencing in primary human cells in the absence of any viral-gene expression. We identified a CTCF motif cluster 5' to the latency promoter and a known long-term regulatory region as important elements for vigorous transgene expression fro ...[more]
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