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Towards an HIV-1 cure: measuring the latent reservoir.

ABSTRACT: The latent reservoir (LR) of HIV-1 in resting memory CD4(+) T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.

SUBMITTER: Bruner KM

PROVIDER: S-EPMC4386620 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Towards an HIV-1 cure: measuring the latent reservoir.

Bruner Katherine M KM Hosmane Nina N NN Siliciano Robert F RF

Trends in microbiology 20150305 4

The latent reservoir (LR) of HIV-1 in resting memory CD4(+) T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. H ...[more]

PMID: 25747663

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