Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes.

ABSTRACT: The discovery of cytosolic RNA granule (RG) component proteins associated with human cataract has initiated investigations on post-transcriptional mechanisms of gene expression control in the lens. Application of established mouse lens epithelial cell lines (LECs) can provide rapid insights on RG function in lens cells, especially because mouse mutants in several RG components are not available. However, although these LECs represent potential reagents for such analyses, they are uncharacterized for lens gene expression or RG formation. Therefore, a detailed molecular and cellular characterization of three permanent mouse LECs 17EM15, 21EM15 and αTN4 is performed in this study. Comparative analysis between microarray gene expression datasets on LEC 21EM15 and iSyTE lens tissue demonstrates

SUBMITTER: Terrell AM 

PROVIDER: S-EPMC4387128 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature