Dataset Information

Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis.

ABSTRACT: Streptococcus suis causes disease in pigs worldwide and is increasingly implicated in zoonotic disease in East and South-East Asia. To understand the genetic basis of disease in S. suis, we study the genomes of 375 isolates with detailed clinical phenotypes from pigs and humans from the United Kingdom and Vietnam. Here, we show that isolates associated with disease contain substantially fewer genes than non-clinical isolates, but are more likely to encode virulence factors. Human disease isolates are limited to a single-virulent population, originating in the 1920, s when pig production was intensified, but no consistent genomic differences between pig and human isolates are observed. There is little geographical clustering of different S. suis subpopulations, and the bacterium undergoes high rates of recombination, implying that an increase in virulence anywhere in the world could have a global impact over a short timescale.

SUBMITTER: Weinert LA

PROVIDER: S-EPMC4389249 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis.

Weinert Lucy A LA Chaudhuri Roy R RR Wang Jinhong J Peters Sarah E SE Corander Jukka J Jombart Thibaut T Baig Abiyad A Howell Kate J KJ Vehkala Minna M Välimäki Niko N Harris David D Chieu Tran Thi Bich TT Van Vinh Chau Nguyen N Campbell James J Schultsz Constance C Parkhill Julian J Bentley Stephen D SD Langford Paul R PR Rycroft Andrew N AN Wren Brendan W BW Farrar Jeremy J Baker Stephen S Hoa Ngo Thi NT Holden Matthew T G MT Tucker Alexander W AW Maskell Duncan J DJ

Nature communications 20150331

Streptococcus suis causes disease in pigs worldwide and is increasingly implicated in zoonotic disease in East and South-East Asia. To understand the genetic basis of disease in S. suis, we study the genomes of 375 isolates with detailed clinical phenotypes from pigs and humans from the United Kingdom and Vietnam. Here, we show that isolates associated with disease contain substantially fewer genes than non-clinical isolates, but are more likely to encode virulence factors. Human disease isolate ...[more]

PMID: 25824154

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Project description:BackgroundAntimicrobial resistance (AMR) is among the gravest threats to human health and food security worldwide. The use of antimicrobials in livestock production can lead to emergence of AMR, which can have direct effects on humans through spread of zoonotic disease. Pigs pose a particular risk as they are a source of zoonotic diseases and receive more antimicrobials than most other livestock. Here we use a large-scale genomic approach to characterise AMR in Streptococcus suis, a commensal found in most pigs, but which can also cause serious disease in both pigs and humans.ResultsWe obtained replicated measures of Minimum Inhibitory Concentration (MIC) for 16 antibiotics, across a panel of 678 isolates, from the major pig-producing regions of the world. For several drugs, there was no natural separation into 'resistant' and 'susceptible', highlighting the need to treat MIC as a quantitative trait. We found differences in MICs between countries, consistent with their patterns of antimicrobial usage. AMR levels were high even for drugs not used to treat S. suis, with many multidrug-resistant isolates. Similar levels of resistance were found in pigs and humans from regions associated with zoonotic transmission. We next used whole genome sequences for each isolate to identify 43 candidate resistance determinants, 22 of which were novel in S. suis. The presence of these determinants explained most of the variation in MIC. But there were also interesting complications, including epistatic interactions, where known resistance alleles had no effect in some genetic backgrounds. Beta-lactam resistance involved many core genome variants of small effect, appearing in a characteristic order.ConclusionsWe present a large dataset allowing the analysis of the multiple contributing factors to AMR in S. suis. The high levels of AMR in S. suis that we observe are reflected by antibiotic usage patterns but our results confirm the potential for genomic data to aid in the fight against AMR.

Dataset Information

Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis.

Publications

Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis.

Similar Datasets

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