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Average genome size estimation improves comparative metagenomics and sheds light on the functional ecology of the human microbiome.

ABSTRACT: Average genome size is an important, yet often overlooked, property of microbial communities. We developed MicrobeCensus to rapidly and accurately estimate average genome size from shotgun metagenomic data and applied our tool to 1,352 human microbiome samples. We found that average genome size differs significantly within and between body sites and tracks with major functional and taxonomic differences. In the gut, average genome size is positively correlated with the abundance of Bacteroides and genes related to carbohydrate metabolism. Importantly, we found that average genome size variation can bias comparative analyses, and that normalization improves detection of differentially abundant genes.

SUBMITTER: Nayfach S

PROVIDER: S-EPMC4389708 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Average genome size estimation improves comparative metagenomics and sheds light on the functional ecology of the human microbiome.

Nayfach Stephen S Pollard Katherine S KS

Genome biology 20150325

Average genome size is an important, yet often overlooked, property of microbial communities. We developed MicrobeCensus to rapidly and accurately estimate average genome size from shotgun metagenomic data and applied our tool to 1,352 human microbiome samples. We found that average genome size differs significantly within and between body sites and tracks with major functional and taxonomic differences. In the gut, average genome size is positively correlated with the abundance of Bacteroides a ...[more]

PMID: 25853934

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